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Abstract, 2016, Guizhou Academy of Agricultural Sciences

An edible mushroom, Pleurotus ostreatus (oyster mushroom) has a worldwide cultivation and is popular in Thailand where rubber sawdust is traditionally used in its cultivation. However, rubber sawdust is a relatively expensive substrate that affects the price of P. ostreatus production. Six different grain media were tested for spawn production. The best spawn production was in Sorghum bicolor (sorghum) media. Five different agricultural wastes for promoting mycelium growth for spawn production were tested. This was followed by sorghum mixed with corn cobs (16.8317 mm/day), and sorghum mixed with rice husks (11 mm/day). Agricultural wastes for P. ostreatus cultivation were also investigated. A comparative study using rubber sawdust, versus rubber sawdust mixed with different supplements of rice straw, rice husks or corncobs to grow P. ostreatus was carried out. The fruiting bodies of P. ostreatus were produced at room temperature and 70–80% humidity. The first primordia and fruiting bodies of P. ostreatus appeared on day 30 after inoculation. The optimal substrate to cultivate P. ostreatus is sawdust + rice husks with an average wet weight harvest of 277.50 ± 79.74 g in a 40 day production cycle.

Key words – alternative substrates – basidiomycota – optimal condition – Pleurotaceae